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zebrafish flii  (GenScript corporation)

 
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    GenScript corporation zebrafish flii
    Zebrafish Flii, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zebrafish flii/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    zebrafish flii - by Bioz Stars, 2026-04
    90/100 stars

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    zebrafish flii  (GenScript corporation)
    90
    GenScript corporation zebrafish flii
    Zebrafish Flii, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zebrafish flii/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    zebrafish flii - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    coding sequence of zebrafish flii  (GenScript corporation)
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    GenScript corporation coding sequence of zebrafish flii
    A–F Interaction <t>of</t> <t>NLRP1</t> with LRRFIP1 and <t>FLII</t> in K562 cells (A, D–F), HEK293 cells (B) and M‐PBMCs (C) assayed by co‐immunoprecipitation assays and PLA (D–F). Endogenous proteins (A, C–F) and transfected FLAG‐tagged full‐length NLRP1, NLRP1ΔCARDΔPYD, NLRP1_S1213A and UPA‐CARD (B) were analyzed. The number of interactions per cell (E) and the percentage of cytosolic interactions (F) are shown ( n = 100 cells). Data are shown as the means ± SEM. P values were calculated using one‐way ANOVA and Tukey's multiple range test. n.s., non‐significant; **** P < 0.0001. #, non‐specific band. Source data are available online for this figure.
    Coding Sequence Of Zebrafish Flii, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coding sequence of zebrafish flii/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    coding sequence of zebrafish flii - by Bioz Stars, 2026-04
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    A–F Interaction of NLRP1 with LRRFIP1 and FLII in K562 cells (A, D–F), HEK293 cells (B) and M‐PBMCs (C) assayed by co‐immunoprecipitation assays and PLA (D–F). Endogenous proteins (A, C–F) and transfected FLAG‐tagged full‐length NLRP1, NLRP1ΔCARDΔPYD, NLRP1_S1213A and UPA‐CARD (B) were analyzed. The number of interactions per cell (E) and the percentage of cytosolic interactions (F) are shown ( n = 100 cells). Data are shown as the means ± SEM. P values were calculated using one‐way ANOVA and Tukey's multiple range test. n.s., non‐significant; **** P < 0.0001. #, non‐specific band. Source data are available online for this figure.

    Journal: EMBO Molecular Medicine

    Article Title: ZAKα / P38 kinase signaling pathway regulates hematopoiesis by activating the NLRP1 inflammasome

    doi: 10.15252/emmm.202318142

    Figure Lengend Snippet: A–F Interaction of NLRP1 with LRRFIP1 and FLII in K562 cells (A, D–F), HEK293 cells (B) and M‐PBMCs (C) assayed by co‐immunoprecipitation assays and PLA (D–F). Endogenous proteins (A, C–F) and transfected FLAG‐tagged full‐length NLRP1, NLRP1ΔCARDΔPYD, NLRP1_S1213A and UPA‐CARD (B) were analyzed. The number of interactions per cell (E) and the percentage of cytosolic interactions (F) are shown ( n = 100 cells). Data are shown as the means ± SEM. P values were calculated using one‐way ANOVA and Tukey's multiple range test. n.s., non‐significant; **** P < 0.0001. #, non‐specific band. Source data are available online for this figure.

    Article Snippet: The coding sequence of zebrafish flii (accession number NM_001257146.1) and human NLRP1‐FLAG (accession number NM_033004.4) (wild type, S107A and S107D) were synthesized by GeneScript.

    Techniques: Immunoprecipitation, Transfection

    A HEK293T cells were transfected with 300 ng NLRP1‐FLAG, 1 ng ASC‐GFP and the indicated concentrations of FLAG‐LRRFIP1 and FLAG‐FLII plasmids, and the formation of ASC specks was analyzed by fluorescence microscopy 24 h post‐transfection. B, C Representative images of ASC specks. D, E Number of positive ASC specks in cells co‐transfected with LRRFIP1 (D) and FLII (E). Expression of LRRFIP and FLII was confirmed by Western blot using anti‐FLAG and anti‐ACTB antibodies. Data are shown as the means ± SEM ( n = 95–156 cells). P values were calculated using one‐way ANOVA and Tukey's multiple range test. * P < 0.05; ** P < 0.01; *** P < 0.001. Source data are available online for this figure.

    Journal: EMBO Molecular Medicine

    Article Title: ZAKα / P38 kinase signaling pathway regulates hematopoiesis by activating the NLRP1 inflammasome

    doi: 10.15252/emmm.202318142

    Figure Lengend Snippet: A HEK293T cells were transfected with 300 ng NLRP1‐FLAG, 1 ng ASC‐GFP and the indicated concentrations of FLAG‐LRRFIP1 and FLAG‐FLII plasmids, and the formation of ASC specks was analyzed by fluorescence microscopy 24 h post‐transfection. B, C Representative images of ASC specks. D, E Number of positive ASC specks in cells co‐transfected with LRRFIP1 (D) and FLII (E). Expression of LRRFIP and FLII was confirmed by Western blot using anti‐FLAG and anti‐ACTB antibodies. Data are shown as the means ± SEM ( n = 95–156 cells). P values were calculated using one‐way ANOVA and Tukey's multiple range test. * P < 0.05; ** P < 0.01; *** P < 0.001. Source data are available online for this figure.

    Article Snippet: The coding sequence of zebrafish flii (accession number NM_001257146.1) and human NLRP1‐FLAG (accession number NM_033004.4) (wild type, S107A and S107D) were synthesized by GeneScript.

    Techniques: Transfection, Fluorescence, Microscopy, Expressing, Western Blot

    A–H Number of erythrocytes (A), neutrophils (B, F‐H), macrophages (C), and HSPCs (D), and caspase‐1 activity (E) in nlrp1 crispant larvae of 2 dpf obtained by injecting one‐cell stage embryos with standard, nlrp1 and/or flii crRNAs/Cas9 complexes. (G, H) Larvae were also treated by bath immersion with 100 μM of the caspase‐1 inhibitor VX‐765. Representative images of neutrophils in flii crispant larvae using Tg ( mpx:eGFP ) reporter line are shown in (G). Each dot represents one individual and the mean ± SEM for each group is also shown. P values were calculated using Student's t ‐test. n.s., non‐significant; * P < 0.05; ** P < 0.01; **** P < 0.0001. Source data are available online for this figure.

    Journal: EMBO Molecular Medicine

    Article Title: ZAKα / P38 kinase signaling pathway regulates hematopoiesis by activating the NLRP1 inflammasome

    doi: 10.15252/emmm.202318142

    Figure Lengend Snippet: A–H Number of erythrocytes (A), neutrophils (B, F‐H), macrophages (C), and HSPCs (D), and caspase‐1 activity (E) in nlrp1 crispant larvae of 2 dpf obtained by injecting one‐cell stage embryos with standard, nlrp1 and/or flii crRNAs/Cas9 complexes. (G, H) Larvae were also treated by bath immersion with 100 μM of the caspase‐1 inhibitor VX‐765. Representative images of neutrophils in flii crispant larvae using Tg ( mpx:eGFP ) reporter line are shown in (G). Each dot represents one individual and the mean ± SEM for each group is also shown. P values were calculated using Student's t ‐test. n.s., non‐significant; * P < 0.05; ** P < 0.01; **** P < 0.0001. Source data are available online for this figure.

    Article Snippet: The coding sequence of zebrafish flii (accession number NM_001257146.1) and human NLRP1‐FLAG (accession number NM_033004.4) (wild type, S107A and S107D) were synthesized by GeneScript.

    Techniques: Activity Assay